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Strategy of chemical conjugation

Factors governing the course and yield in ehemical conjugation [Pg.224]

The relative rate of intramolecular over intermolecular cross-linking increases at lower protein concentrations. [Pg.224]

Molecular concentration ratio of the 2 molecules to be conjugated (formation of different conjugates follows Poisson distribution). [Pg.224]

The relative reaction rates of the cross-linking agent with the two molecules (formation of homopolymers vs. heteropolymers). [Pg.224]

Effective concentration of the cross-linking agent the active fraction of the reagent depends on a large number of parameters, such as pH, medium, etc. [Pg.224]


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