Stopped-flow iron porphyrin

The rates of ligand binding and ligand dissociation kon and off) can be determined by stopped-flow methods. For example, the kinetics of the binding and release of NO with an iron(III) porphyrin complex was stndied as a function of pH, temperature, and pressure by stopped-flow and laser flash photolysis techniqnes. The diaqua-ligated form of the porphyrin complex binds and releases NO according to a dissociative interchange mechanism based on the positive values of the activation parameters and for the on and off reactions. 

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