Stopped-flow CD and lysozyme folding

One of the earliest kinetic reactions to be followed by CD was the racemization of [Fe(l,10-phenanthroline)3]. This takes place with a half-life of approximately 20 min at room temperature. [Co(l,10-phenanthroline)3] when synthesized under fairly rigorous oxidation conditions (we found chlorine gas was effective (6)) is essentially stable at room temperature. Literature claims of rapid racemization, e.g. (7), are in fact the result of the presence of catalytic amounts of the labile Co(ll) complex electron transfer occurs readily between the different oxidation states of the tris-phenanthroline complexes. 

When enantiomerically pure [Co(l,10-phenanthroline)3] and racemic [Co(l,10-phenanthroline)3] are mixed in the presence of DNA, the DNA causes 

Spectrophotometry and fluorimetry of cellular compartments and Intracellular processes 

Department of Biochemistry and immunology, Cellular and Molecular Sciences Group, St George s Hospital Medical School, Cranmer Terrace, London SW17 ORE 

The main problem with optical measirrements is not the acquisition but rather the interpretation of the data obtained. Straightforward analysis of the results depends on the clarity of the experimental design and the appropriate choice of chromophore. This chapter describes some of the problems that can be addressed by spectroscopic techniques and attempts to give guidance on good experimental design. 

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