Steroid sulphotransferase

Inhibition of steroid sulphatase and sulphotransferase Steroid sulphotransferase catalyses the addition of sulphate to steroidal compounds whilst steroid sulphatase catalyses the reverse reaction. In vitro studies have demonstrated that a metabolite of genistein, 4-ethylphenol, can inhibit sulphotransferase (Harris et al, 2000). Sulphoconjugates of daidzein have also been found to potently inhibit these enzymes in vitro (Wong and... 

Radominska, A., Comer, K. A., Zimniak, P., Falany, J., Iscan, M., and Falany, C. N. (1990) Human liver steroid sulphotransferase sulphates bile acids. Biochem. J. 272, 597-604. 

C. Sulphation Sulphotransferases, e.g. phenol and steroid alcohol sulphotransferase Mainly phenols but also alcohols, amines and thiols ROH + 3 -phosphoadenosine-5 -phosphosulphate (PAPS) R-OSO3H + ADP... 

Sulphate conjugates, once called ethereal sulphates because of their extraction characteristics, are of two major types aryl or alkyl sulphates (which are esters of phenols or alcohols) and steroid sulphates. The sulphate moiety is transferred from the cofactor adenosine-3 -phosphate-S -phosphosulphate (PAPS) to an acceptor by enzymes known as sulphotransferases or sulphokinases. The enzymes which catalyse the formation of PAPS as well as the sulphokinases are in the soluble fraction of liver ... 

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