Stereoselective Production of Substituted Cyclohexylanhydrins

The effect of the enzyme source upon the formation of 4-methoxycyclohexylcyano-anhydrin is summarized below  

Enzyme Source Units/mmol Rx. Time (hr) Conversion (%) Trans/Cis Ratio 

Solvent effects upon the formation and stereoselectivity of 4-methoxycyclohexylanhydrin using the (R)-oxynitrilase enzyme derived from P. amygdalus was also determined by the author and summarized below  

The effect upon formation of 4-methoxycyclohexylanhydrin using either (R)- and (S)-oxynitrilases contrasted with an uncatalyzed cyclohydrin reaction are summarized below  

Substituent effect upon 4-substituted cyclohexylanhydrin formation, illustrated below in Eq. 1, using canna-derived (S)-oxynitrilase in 100 units/mmol on a nitrocellulose matrix in diisopropyl ether is summarized below  

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