Stellacyanin characterization

The type-1 blue copper proteins act as electron carriers azurin, plastocyanin, stellacyanin, umecyanin e.g. They are characterized by a rather strong LMCT (ligand to metal charge transfer) band near 600 nm and by small hyperline coupling constants A in EPR. Copper is bound to two imidazole groups of histidine and to two... 

The third class consists of proteins that are composed of one or more BCB domains fused to a sequence domain(s) characteristic of evolutionarily unrelated protein families. Such a mosaic domain organization has been found in the phytocyanin protein family, stellacyanins, uclacyanins, and the recently characterized dicyanins (Section V) in blood coagulation factor VIII (Section VIII) and in nitrous oxide reductase (Section IX). 

Plantacyanins are strongly basic proteins with an isoelectric point close to 11. First isolated from cucumber in 1974, plantacyanins have since been characterized from various other plant species (Markossian et al., 1974 Aikazyan and Nalbandyan, 1981). They exhibit relatively high sequence identity to stellacyanin and spectroscopic properties similar... 

Co(II) has been the most useful metal probe for the study of BCE The Co(II) derivatives of Ps. aeruginosa azurin (Moratal Mascarell et al., 1993a Piccioli et al., 1995 Salgado et al., 1995), Rhus vernicifera stellacyanin (Fernandez et al., 1997 Vila, 1994 Vila and Fernandez, 1996), Ac. cyclo-clastes pseudoazurin (Fernandez et al., 2001), Thi. ferrooxidans rusticyanin (Donaire et al., 2001), Thi. versutus amicyanin (Salgado et al., 1999), several mutants of azurin (Piccioli et al., 1995 Salgado et al., 1996, 1998a Vila et al., 1997), and the M99Q mutant of amicyanin (Diederix et al., 2000) have been prepared, and their H NMR spectra have been characterized. 

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