Standard Protein Digests

In vitro protein digestibility requires preparation of a treated sodium caseinate standard. Because of the empirical nature of these determinations, it is critical that results for the so-... 

We first evaluated the performance of the device described in Figure 2.5 for the analysis of protein digests. Different standard protein tryptic digests... 

Extensive evaluation of existing in vitro and in vivo methods in foods indicates that the rat balance method is the most suitable practical method for predicting protein digestibility by humans. Therefore, when human balance studies cannot be used, the standardized rat fecal-balance method of [119] or [109] is recommended. 

Glass microfluidic chips with inserted nano-ESI emitters, electroosmotic pumping capillaries, or capillary transfer lines inserted in the liquid sheath interface of a Q-TOF instrument, were also used for the CE analysis of standard peptide/protein digests and of gel-isolated proteins from complex cellular extracts (H. influenza, P. Some of these chips enabled sam-... 

FIGURE 3 Polyacrylamide gel electrophoresis of native and digested phycocyanm. (1) Native and (2) digested (pancreatic digestion) phycocyanms were electrophoresed on a slab gel with a 5-20% linear polyacrylamide gradient in the presence of SDS molecular mass of standard proteins (3) data are typical of three independent experiments. 

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