Stabilizing agents trehalose

Rudolph, A.S., Crowe, J.H. Crowe, L.M. (1986). Effects of three stabilizing agents - proline, betaine and trehalose on membrane phospholipids. Archives of Biochemistry and Biophysics, 245, 134-43. 

Certain additives, salts, and bulking agents may be added primarily to improve vaccine stability upon storage (19,20). These excipients such as mannitol, glycine, and trehalose have a direct impact on the stability of the polypeptide or conjugate and are investigated for this purpose. 

Hinrichs et al. [1.150] compared inulin of various degrees of polymerization with trehalose as glass-forming agents. Inilin above a certain degree of polymerization, DPn/DPw > 5.5/6.0, and trehalose stabilize alkaline phosphatase equally well. The Tg and T values for inulin of <5.5/6.0 where higher than those for trehalose. 

A dilute solution of insulin in an appropriate aqueous medium, such as PBS (pH 7.4) or citrate buffer (pH 3 or 5), is prepared (see Note 1). Additives, such as lactose, trehalose, mannitol, or DPPC, are added as needed. These additives serve many purposes as bulking agents (4) to improve processing (4), to improve stability (6). and to increase bioavailability of the administered drug (4,7). 

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