Spinning cup sequencer

In order to overcome some solubility problems, Garrick and Sloan have suggested that small peptides may be sequenced in the spinning cup type of automatic sequencer after covalent attachment to cytochrome c. The acetylated A terminus of the cytochrome minimizes the risk of confusion due to its own... 

Having determined the structure of the SH-peptide we started investigating the fraction of peptides soluble in 0.1% TFA. The peptides were separated by reversed phase HPLC (Figure 4). Save for one exception all the peptides were obtained in pure state and their sequences were established in a solid-phase protein sequencer in the case of peptides with C-terminal lysine, or in the spinning cup sequenator in the case of peptides C-terminated with arginine (Figure 1,3). 

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