Spin-labeling methodology, electron

The elucidation of PrP structure and function often necessitates the incorporation of biophysical probes. These monitors can be fluorescent dyes which enable one to study protein function both in vivo and in vitro by modem fluorescence methods. The incorporation of nuclear magnetic resonance (NMR) sensitive isotopes or spin labels opens the route to NMR and electron paramagnetic resonance (EPR) methodology. In the following these three methods will be briefly discussed in respect of their application in prion research. 

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