Spin label relaximetry

Fig. 7. Results from spin label relaximetry on spin-labeled site-directed mutants of bacteriorhodopsin. The highlighted gray region on transmembrane helices D and E and the interconnecting loop indicate the sequential spin-labeled positions. The positions marked by boxes show sensitivity to CROX, thus locating the precise residues on the interhelical loop. The circled positions show sensitivity to O2, and this locates the region of helix E that points away from the protein and into the membrane bilayer. The remaining residues are buried within the protein interior.

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