Spiegelmer approach

The insufficient stability of RNA, mentioned above, limits the use of RNA aptamers. However, some established techniques yield nuclease-resistant aptamers. One alternative is called the Spiegelmer approach [29], another method involves incorporation of 2 -methoxy purine nucleotides [30]. 

Vater, A. Jarosch, F. Buchner, K. Klussmann, S. Short bioactive spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach Tailored-SELEX. Nucl. Acids Res. 2003, 31 (21), el30. 

Leva, S. et al., GnRH binding RNA and DNA spiegelmers a novel approach toward GnRH antagonism, Chem. Biol, 9,351,2002. 

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