Spectroscopy bandshape

A limitation of the application of luminescence spectroscopy to the analysis of real samples is its lack of specificity owing to similarities in spectral bandshapes and spectral positions of the luminescence spectra of many compounds. An obvious solution to this problem is the separation of the analytical sample s interfering constituents from each other before quantitation by fluorescence. High-performance liquid chromatography (HPLC) and related separation methods can be coupled to fluorescence spectroscopy to take advantage of the sensitivity of the spectroscopic method and the specificity of the separation method. 

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