Spectroscopically orthogonal spin labels and distance

EPR-based techniques to measure nanometre range distances are nowadays recognized as a valuable tool in studies of structure and conformational changes of biomacromolecules. A typical approach uses some type of nitroxide-based spin labels, which are selectively attached to specific sites.The distances between pairs of these spin labels are then measured by some appropriate pulse EPR technique,most commonly, by the dead-time-free 4-pulse double electron-electron resonance (DEER) experiment.  

Laboratory of Physical Chemistry, Department of Chemistry and Applied Bioscience, ETH Zurich, Switzerland. E-mail maxim.yulikov phys.chem.ethz.ch 

Electron Paramag. Reson., 2015, 24, 1-31 1 The Royal Society of Chemistry 2015 

1 Brief overview of the spectroscopically-selective spin label pairs for DEER spectroscopy 

The idea of having two non-identical spin labels that can be distinguished spectroscopically appeared rather early after DEER (sometimes also called PELDOR) and related techniques have attracted considerable attention. It has been demonstrated that labelling 

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Spectroscopically orthogonal spin labels and distance measurements in biomolecules... 

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