Spectral imaging advantages

Robust multivariate classification can require large numbers of samples in order to characterize the covariance of the component distributions. One of the major advantages of spectral imaging over single-point spectroscopy is the ready... 

In this chapter, we talked about the advantages of using spectral imaging in the NIR spectral region compared with spectral imaging in the mid-IR spectral region (the former can more easily analyze thicker samples and use less expensive, more... 

Unfortunately, the diffraction limit does not permit spectral images to be collected from cells unless the near-field advantage is exploited. However, we have mapped mytotic cells using Raman imaging microspectroscopy, and have reported spectral images of mytotic cells in the telophase and metaphase.17... 

With each method of FRET estimation there are advantages and disadvantages. Here, specifically the three most widely used other FRET-imaging techniques besides FRET-FLIM i) acceptor-bleaching, ii) filter-FRET and iii) spectral imaging are briefly discussed in relation to FLIM. Also elsewhere, reviews and experimental studies have appeared comparing FLIM with other FRET-microscopy techniques [61,63,108]. 

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