Spectra myelin

The importance of P0 in PNS myelin has been clearly demonstrated. In P0 gene knockout experiments in mice [40], severe hypomyelination and a virtual absence of compact myelin in the PNS is observed. In humans, there are two disease states associated with mutations in the P0 gene Charcot-Marie-Tooth type I disease (see Ch. 38) and Dejerine-Sottas disease, both dysmyelinating diseases that exhibit a spectrum of severity depending on the particular mutation. 

Figure 2.11. The dependence of the position of the fluorescence spectrum maximum on excitation wavelength for tryptophan in a model medium (glycerol) at different temperatures (a) and singletryptophan proteins (b). 1, Whiting parvalbumin, pH 6.S in the presence of Ca2+ ions 2, ribonuclease Th pH 6.5 3, ribonuclease C2, pH 6.5 4, human serum albumin, pH 7.0, +10"4 M sodium dodecyl sulfate 5, human serum albumin, pH 3.2 6, melittin, pH 7.5, +0.15 M NaCl 7, protease inhibitor IT-AJ from Actinomyces janthinus, pH 2.9 8, human serum albumin, pH 7.0 9, -casein, pH 7.5 10, protease inhibitor IT-AJ, pH 7.0 11, basic myelin protein, pH 7.0 12, melittin in water. The dashed line is the absorption spectrum of tryptophan.

The FT-IR spectrum of MBP protein after reconstitution in myelin (with all proteins removed) was also recorded. The results of deconvolution and band-fitting the amide I band of MBP in myelin are shown in Figure 6.4b, with the corresponding data for. the frequency and the fractional area of each component being listed in Table 6.4b. 

Yamamoto Y, Sato Y, Taniguchi K (2007) Distribution of TRPVl- and TRPV2-immunoreactive afferent nerve endings in rat trachea. 1 Anat 211 775-783 Yu 1 (2000) Spectrum of myelinated pulmonary afferents. Am 1 Physiol Regul Integr Comp Physiol 279 R2142-2148... 

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