Soyasaponin III

In order to prove the hypothesis obtained from HPLC profile analysis, antihepatotoxic effects of OGs obtained from Abri Herba were tested [30]. The tested OGs were representative ones, soyasaponin I (soyaspogenol B OG, 1) and kaikasaponin III (sophoradiol OG, 21). The antihepatotoxic activity was studied on liver injury induced by CC14 in primary cultured rat hepatocytes [31], Fig. (5). 

Fig. (5). Effects of Some OGs on Primary Cultured Rat Hepatocytes injured with CC14 Effects of glycyrrhizin (GL, triangles), kaikasaponin III (2 1, Kaika III, circles) and soyasaponin I (1, Soya I, squares) on CCI4 (SmM)-induced cytotoxicity in primary cultured rat hepatocytes. The marker of liver injury is the aspartate aminotransferase (AST), measured in IU (International Units)/ . Data are the mean S.D. for three independent cell preparations.

Glycine soya is a wild-type relative to the cultivated soybean (Glycine max). We isolated four known OGs, soyasaponins 1(1), II (3), III (6) and IV (8) from the aerial parts of this plant [37]. The results of hepatoprotective actions of these OGs are shown in Fig. (7) [37]. 

Fig. (7). Hepatoprotective Actions of Soyasaponins I - IV toward in vitro Immunological Liver Injury on Primary cultured Rat Hepatocytes Control (Cont.) is the value of hepatocytes which were not administered the antiserum. Reference (Ref.) value were administered the antiserum and not treated with Soyasaponins I (A), II (B), III (C), IV (D). Significantly different from Ref., effective p <0.01.

Table 4. Hepatoprotective and Hepatotoxic Activity of for Hydrolytic Products of Kaikasaponin III and Soyasaponin I...

Fig.2. Effects of Glycyrrhizin (GL), Kaikasaponin III (Kaika III) and Soyasaponin I (Soya I) on CCI4 (5mM)-Induced Cytotoxicity In Primary Cultured Rat Hepatocytes. The data are the mean S.D. for 3 independent cell preparations.

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