Solvent systems sample streaking

Figure 12.3 Sample application procedure using guide strips for PLC. Up to 1 mg of lipid was streaked across the center 10 cm of a 20 x 20-cm analytical plate, which was developed in the dual solvent system of Skipski et al. (Chapter 15). Major bands from the organism contained sterol (II) and phospholipid (I). The band labeled "blank" was lipid negative. Lanes 1 and 4 of the edge strips (5 cm wide) were spotted with neutral lipid standards, lanes 2 and 3 with sample. The edge strips were cut and visualized with 10% phosphomo-lybdic acid in ethanol and were then matched with the center to determine the position of the bands.

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