Solution-phase synthesis size exclusion chromatography

Boger DL, Goldberg J, Andersson CM, Solution phase combinatorial synthesis of biaryl libraries employing heterogeneous conditions for catalysis and isolation with size exclusion chromatography for purification, J. Org. Chem., 64 2422-2429, 1999. 

Solution phase supports have the problem of product recovery. An alternative approach is to couple the substrate to a water-soluble polymer that can be removed from solution by precipitation of the polymer or size exclusion chromatography. Water-soluble supports have been used in the enzymatic synthesis of pseudo-GM3 (118). 

The use of solid supports which can be dissolved in many solvents allows one to perform the reactions for library synthesis in a homogeneous medium, but also to separate the intermediates from the reaction solution with common purification techniques (precipitation, crystallization, size-exclusion chromatography, and so on). We will present two examples related to liquid-phase combinatorial syn-... 

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