Solid phase extraction contaminant elimination from

Proper sampling procedures have to be applied to obtain representative samples from analyzed biological material since mycotoxins are not homogenously distributed in contaminated grain stocks. Sample preparation usually involves a few cleanup steps to eliminate compounds present in the matrix that might coelute with the mycotoxins. The cleanup methods may involve either liquid-liquid extraction (LLE), solid-phase extraction (SPE) on columns that contain various types of solid phases, or immunoaffinity columns [39,42,43]. The recovery achieved with a cleanup method is critical for the final results. 

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