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Sodium pantothenate

Various labeled degradation products of pantothenic acid and coenzyme A are known. Both (l- C)-sodium pantothenate (27) and (R)-(l- C)-panthenol are... [Pg.60]

Figure 2. Time course for CoA synthesis by dried cells of B. ammoniagenes. CAJ Synthesis from pantothenic acid reaction mixture (1 mL) containing 5 [imol sodium pantothenate, 10 /imol cysteine, IS /imol ATP, 10 y.mol magnesium sulfate, ISO pmol potassium phosphate buffer, pH 6.0, and 100 mg dried cells of B. ammoniagenes was incubated at 37°C with (a) or without (b) 2 mg of sodium hurylbenzenesulfonate. A mixture without sodium pantothenate (c) was used as a control run. (B) Synthesis from pantetheine the reaction conditiotK were the same as those in (A) except that an equimolar amouni of pantethine was used in place of sodium pantothenate. Figure 2. Time course for CoA synthesis by dried cells of B. ammoniagenes. CAJ Synthesis from pantothenic acid reaction mixture (1 mL) containing 5 [imol sodium pantothenate, 10 /imol cysteine, IS /imol ATP, 10 y.mol magnesium sulfate, ISO pmol potassium phosphate buffer, pH 6.0, and 100 mg dried cells of B. ammoniagenes was incubated at 37°C with (a) or without (b) 2 mg of sodium hurylbenzenesulfonate. A mixture without sodium pantothenate (c) was used as a control run. (B) Synthesis from pantetheine the reaction conditiotK were the same as those in (A) except that an equimolar amouni of pantethine was used in place of sodium pantothenate.
Figure 5. Effect of temperature on the stability of immobilized cells. The gel-entrapped dried cells (a) were incubated in O.OIM potassium phosphate buffer, pH 7.0, for 3 hr Figure 5. Effect of temperature on the stability of immobilized cells. The gel-entrapped dried cells (a) were incubated in O.OIM potassium phosphate buffer, pH 7.0, for 3 hr <a various temperatures, as indicated. After filtration, each gel (1.88 g) was incubated with 20 [imol sodium pantothenate, 100 [imol cysteine, ISO nmol ATP, 100 nmol magnesium sulfate, 1500 nmol potassium phosphate buffer, pH 6.5, 20 nmol CTP, and 10 mg sodium lauryl-sulfate in a total volume of 10 mL. The reaction was carried out at 37 C for 5 hr with shaking. Free, dried cells (b) were used as cotUrol. (A) Accumulation of Co A (B) consumption of pantothenate.
Figure 7. Time course of CoA synthesis in the presence of CoA. (A). Synthesis from pantothenic acid-, the reaction teas carried out with 4 ftmol (a) or 2 funol (b) CoA. Other conditions were the same as those described in Figure 2(A), except for addition of 2 mg sodium laurylsulfate. The mixture without CoA (c) was used as a control run. (B) Syrtthesis from P-pantothernc acid the reaction conditions were the same as those described in (A) except that sodium pantothenate was replaced with P-pantotheruc acid. Symbols are the same as those in (A). Figure 7. Time course of CoA synthesis in the presence of CoA. (A). Synthesis from pantothenic acid-, the reaction teas carried out with 4 ftmol (a) or 2 funol (b) CoA. Other conditions were the same as those described in Figure 2(A), except for addition of 2 mg sodium laurylsulfate. The mixture without CoA (c) was used as a control run. (B) Syrtthesis from P-pantothernc acid the reaction conditions were the same as those described in (A) except that sodium pantothenate was replaced with P-pantotheruc acid. Symbols are the same as those in (A).
Figure 8. Synthesis of CoA on an immobilized cell column. (A) Single column system a substrate mixture composed of sodium pantotheruite (2,5 fimol/mL), cysteine (10 nmoUmL), ATP (15 fxmoHmL), magnesium sulfate (10 imol/mL), potassium phosphate buffer, pH 6.5 (150 nmol/mL), and sodium laurylsulfate (1 mg/mL) teas applied to a column (1 X 20 cm) of gel-entrapped dried cells. The reaction was carried out at 34°C with a flow rate of SV = 0.1-O.2 hr h (B) Separated column system a substrate mixture composed of sodium pantothenate (2.5 /imol/mL), ATP (7.5 ixmol/mL), magnesium sulfate (10 nmol/mL), potassium phospate buffer, pH 6.5 (150 fimol/mL), and sodium laurylsulfate (1 mg/mL) was applied to the top of the column (1 X 10 cm). Solution (about 20 mh) passed through the column at a flow rate of SV = 0.1-0.2 hr t was collected every day. To the solution (20 mL), 200 nmol cysteine and 150 fimol ATP were added, which was then reacted at the bottom of the column (1 X 10 cm) with a flow rate of SV = 0.1-0.2 hr to yield CoA. The reaction temperature was 34°C. Consumption of pantothenic acid was checked both at the top (b) and bottom (a) columns. Figure 8. Synthesis of CoA on an immobilized cell column. (A) Single column system a substrate mixture composed of sodium pantotheruite (2,5 fimol/mL), cysteine (10 nmoUmL), ATP (15 fxmoHmL), magnesium sulfate (10 imol/mL), potassium phosphate buffer, pH 6.5 (150 nmol/mL), and sodium laurylsulfate (1 mg/mL) teas applied to a column (1 X 20 cm) of gel-entrapped dried cells. The reaction was carried out at 34°C with a flow rate of SV = 0.1-O.2 hr h (B) Separated column system a substrate mixture composed of sodium pantothenate (2.5 /imol/mL), ATP (7.5 ixmol/mL), magnesium sulfate (10 nmol/mL), potassium phospate buffer, pH 6.5 (150 fimol/mL), and sodium laurylsulfate (1 mg/mL) was applied to the top of the column (1 X 10 cm). Solution (about 20 mh) passed through the column at a flow rate of SV = 0.1-0.2 hr t was collected every day. To the solution (20 mL), 200 nmol cysteine and 150 fimol ATP were added, which was then reacted at the bottom of the column (1 X 10 cm) with a flow rate of SV = 0.1-0.2 hr to yield CoA. The reaction temperature was 34°C. Consumption of pantothenic acid was checked both at the top (b) and bottom (a) columns.
P-Alanine, N-(2,4-dihydroxy-3,3-dimethyl-1 -oxobutyl)-, monosodium salt (R)-. See Sodium pantothenate... [Pg.134]

Ritapan DL 50%] Ritapan DL. See DL-Panthenol Ritapan NAP. See Sodium pantothenate Ritapan TA. See Panthenyl triacetate R.i.T.A. d-Panthenol. See D-Panthenol R.I.T.A. dl-Panthenol. See DL-Panthenol Ritapeg ISO DS. See PEG-150 stearate Ritapeg 400 DS. See PEG-8 dIstearate Ritaphenone 3. See Benzophenone-3 Ritaquat Q. See Steartrimonlum hydroxyethyl hydrolyzed collagen... [Pg.3846]

Sodium pantothenate Sodium phosphate dibasic anhydrous Sodium phosphate dibasic heptahydrate Sodium phosphate tribasic Sorbitol... [Pg.5090]

Hydrolyzed fibronectin Hydrolyzed silk Isopentyidiol Lactamide DGA Lauryl PCA Phytantriol Polyquaternium-51 Quaternium-22 Saccharide isomerate Silk amino acids Sodium pantothenate Sodium polyaspartate Soluble collagen Tricontanyl PVP humectant, skin conditioners Wheat amino acids humectant, skin soothing preps. [Pg.5368]

Vitamin K Wheat (Triticum vulgare) germ Zinc gluconate Zinc methionine sulfate Zinc oxide Zinc stearate Zinc sulfate Zinc sulfate heptahydrate nutrient, gelatin capsules Retinyi paimitate nutrient, geriatric food Lactose monohydrate nutrient, hair care Sodium pantothenate nutrient, health food Lactose monohydrate Octacosanol nutrient, horticulture Magnesium sulfate heptahydrate nutrient, infant formulas Ferric pyrophosphate Ferrous fumarate Ferrous lactate Ferrous sulfate heptahydrate Inositol... [Pg.5485]

Sodium ascorbate Sodium gluconate Sodium pantothenate Sodium phosphate dibasic heptahydrate Thiamine HCI L-Threonine Tocopherol D-a-Tocopherol pL-a-Tocopherol d-a-Tocopheryl acetate dl-a-Tocopheryl acetate DL-a-Valine L-Valine Zinc gluconate Zinc methionine sulfate Zinc sulfate Zinc sulfate heptahydrate nutrient, plant... [Pg.5486]

Serum protein Sodium pantothenate nutrient, soil... [Pg.5486]

Propazine Terbuthylazine Trietazine C9Hi6NNaOs Sodium pantothenate C9Hi N2... [Pg.7062]

Calcium and sodium pantothenate can be chromatographed following derivatiza-tion, degradation, or a combination of the two. Derivatization is mostly based on the determination of the corresponding acetates or trimethylsilyl ethers. The determination of pantothenic acid and/or pantothenates as acetates (72) requires esterification of the carboxyl group prior to acetylation of the hydroxyl function. [Pg.587]

Figure 23 Determination of sodium pantothenate and panthenol as trimethylsilyl derivatives. Detector FID column glass, 2440 X 4 mm stationary phase 5% SE-30 solid support Gas Chrom Q (100/120 mesh) temperatures injector 270°C, column 185°C, detector 270°C peak identification (1) trimethylsilyl derivative of panthenol (2) trimethylsilyl derivative of pantothenic acid (sodium pantothenate). (From Ref. 73.)... Figure 23 Determination of sodium pantothenate and panthenol as trimethylsilyl derivatives. Detector FID column glass, 2440 X 4 mm stationary phase 5% SE-30 solid support Gas Chrom Q (100/120 mesh) temperatures injector 270°C, column 185°C, detector 270°C peak identification (1) trimethylsilyl derivative of panthenol (2) trimethylsilyl derivative of pantothenic acid (sodium pantothenate). (From Ref. 73.)...
Vitamin B5 occurs in three biologically active forms in foods [1] pantothenic acid, coenzyme A (CoA), and acyl carrier protein (ACP). Calcium or sodium pantothenate are the forms generally used as supplements in infant formula [4], The total quantification of vitamin B5 requires the release of pantothenic acid from CoA and ACR Since it consists of pantoic acid linked through an amide linkage to p-alanine, chemical hydrolysis cannot be used. The only alternative to free pantothenic acid from CoA is the digestion with a number of enzymes (pepsin, alkaline phosphatase, pantetheinase) nevertheless, this treatment is unable to release the vitamin from ACP [27,28]. For the extraction of free pantothenic acid from milk and calcium pantothenate from infant formula an acidic deproteination is often used, followed by centrifugation and filtration [29,30]. [Pg.484]

Pantothenic acid or its salts are added to foods only occasionally. Calcium and sodium pantothenate are more stable and less... [Pg.383]

See other pages where Sodium pantothenate is mentioned: [Pg.487]    [Pg.1151]    [Pg.402]    [Pg.319]    [Pg.88]    [Pg.4084]    [Pg.5367]    [Pg.6159]    [Pg.565]   
See also in sourсe #XX -- [ Pg.4 , Pg.4 , Pg.401 , Pg.402 ]

See also in sourсe #XX -- [ Pg.401 , Pg.402 ]



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Pantothenate

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