Smithying, also

Plots of these elements on the house floor (Fig. 6.24) show clear differences and patterns. The results of the elemental analysis demonstrate different areas in the two structures. The smithy has low Ca, Cu, Fe, and K, while values for Mg, Mn, and Pb are above average. The two rooms in the smithy also show differences. In the residential structure, the stable areas are generally average for most of the elements, although Fe, K, Mn, and Zn are slightly higher in some of the samples. Zones of movement in the structures, doorways and entrance area, have lower concentrations of residues in the sediment, due to the erosion of the surface from movement. 

After treatment of purified Hp of type 2-1 and 2-2 with a combination of urea and thioglycolate, Smithies and Connell (S8) found that these haptoglobins had been depolymerized. That the different Hp lines represent different degrees of polymerization is also supported by the findings of Kluthe and Isliker (Kl). 

The starch is boiled with 0.007 M phosphate buffer, pH 7.5, the electrode vessels are filled with 0.03 M phosphate buffer of pH 7.0, which is also used for the bridges. Smithies simple plastic trays (S5) for horizontal starch gel electrophoresis will give results clear enough for routine work. 

For a discussion of which positions are favorable, see Burke Silks Strickland Tetrahedron Lett. 1988,29,2761. Kabasakalian Townley J. Am. Chem. Soc. 1962,84, 2711 Akhtar Barton Sammes J. Am. Chem. Soc. 1965, 87, 4601. See also Nickon Ferguson Bosch Iwadare J. Am. Chem. Soc. 19T7, 99, 4518 Barton Hesse Pechet Smithy. Chem. Soc., Perkin Trans. I 1979, 1159 Green Boyle Vairamani Mukhopadhyay Saunders Bowen Allinger J. Am. Chem. Soc. 1986,108, 2381. 

Tattenden, G. Roberts, L. Blake, A.J. J. Chem. Soc., Perkin Trans. 11998, 863 Batsanov, A. Chen, L. Gill, G.B. Pattenden, G. J. Chem. Soc., Perkin Trans. 11996, 45. Also see, Pattenden, G. Smithies, A.J. Tapolczay, D. Walter, D.S. ]. Chem. Soc., Perkin Trans. 1 1996, 1 for a related reaction that generates a bicyclic species from an initially generated alkyl radical. 

Also, Hodson et al. (H17) applied Smithies technique for the characterization of alkaline phosphatases from different tissues (intestine, liver, bone, etc.). In their report they questioned the contention that the osseous system is the main contributing source of human plasma alkaline phosphatase (H17), and preferred liver alkaline phosphatase as the main fraction of serum alkaline phosphatase. Hodson et al. (H17) observed the occasional presence of alkaline phosphatase at the intestinal region also in sera after electrophoresis. 

However, Borden and Smithi have found that treatment of the p-nitro-phenyl esters of 2 -deoxyribonucleoside 3 - and 5 -phosphates and of ribo-nucleoside 5 -phosphates with potassium fert-butoxide in methyl sulfoxide affords the corresponding nucleoside 3 5 -cyclic phosphates in excellent yields. Other phosphorus-activated nucleotides, such as nucleoside 5 -phosphorofluoridates, 5 -(2,4-dinitrophenyl phosphate)s and 5 -(PS-P " diphenyl pyrophosphate) s, were also cyclized to nucleoside 3 5 -cyclic... 

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