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Small affinity chemical proteomics

Besides protein microarray proteome profiling, the term chemical proteomics (also chemoproteomics or pull-downs ) is mostly used in reference to the application of affinity chromatography protein purification when small molecules are the bait, and liquid chromatography separation of peptides precludes mass spectrometry as the universal readout (LC-MS/MS). [Pg.81]

Fig. 1 SILAC-based affinity chromatography for identification of the target proteins of small molecules, (a) Generation of two cell populations differentially labeled with light or heavy amino acids, (b) Employment of the metabolically labeled cell lysates from (a) in affinity-based chemical proteomics... Fig. 1 SILAC-based affinity chromatography for identification of the target proteins of small molecules, (a) Generation of two cell populations differentially labeled with light or heavy amino acids, (b) Employment of the metabolically labeled cell lysates from (a) in affinity-based chemical proteomics...

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See also in sourсe #XX -- [ Pg.81 , Pg.82 , Pg.83 ]




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