Size Exclusion chromatography axial dispersion

Lederer, K., Imrich-Schwarz, G., and Dunky, M., Simultaneous calibration of separation and axial dispersion in size exclusion chromatography coupled with light-scattering, J. Appl. Polym. Sci., 32, 4751, 1986. 

In ideal size-exclusion chromatography (SEC), fractionation is exclusively by hydrodynamic volume. Due to axial dispersion, however, a whole distribution of hydrodynamic volumes (and, therefore, of molecular weights) is instantaneously present in the detector cell. Under these conditions, it is assumed that the mass chromatogram w(V) (i.e., the instantaneous mass w versus the elution time or elution volume V) is a broadened version of a true (or corrected) mass chromatogram w V), as follows [1] ... 

Schndll-Bitai, I. The direct determination of axial dispersion in size exclusion chromatography based on Poissonian chain length distributions. Chromatographia 2003, 58, 375-380. 

Jackson, C. Evaluation of the effective volume shift method for axial dispersion corrections in multi-detector size exclusion chromatography. Polymer 1999, 40, 3735-3742. 

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