Site-specific ligand fragments

In the online HX-MS/MS workflow, ETD fragmentation can be set up for all peptides in order to obtain as much site-specific HX information as possible, or alternatively, a targeted HX-ETD approach [47, 57] can be used to delineate site-specific HX only for selected peptides that display altered deuterium uptake upon ligand binding or across different protein states (Figure 8.9). 

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