Sink float fraction collection

In the procedure described here a method analogous to the collection of distillation cuts was employed. Ihe shale was subjected to a low specific gravity and the float fraction collected. Ihe sink fraction was subjected to an incrementally higher specific gravity and the float fraction collected. The procedure was repeated until very little material remained. In this manner a series of samples, differing primarily by organic to rock ratio, was collected. 

In plasma, Lp(a) moves with a pre-beta-1 mobility (B8, B9) and has been referred to as the sinking pre-beta fraction, as it shares the mobility of VLDL yet does not float at the same density in the ultracentrifuge. Serum electrophoresis followed by a lipid staining with Oil Red O or Fat Red should be a convenient method for detection of elevated Lp(a) levels. Sample collection and preservation are, however, critical parameters in this assay (B26, K9). 

Carrying out flotation in a flotation machine by adding air (or gas). Hydrophobic particles adhere to air bubbles generated in the machine, float to the slurry sitrface, and are collected as a froth fraction, while the hydrophilic particles remain in the cell and discharge as a cell (sink) fraction. 

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