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Single ribozyme-substrate complexes

Figure 6.17 Docked states have a strong memory effect, as indicated by FRET time traces of single rlbozyme-substrate complexes, (a) Memory effect of the undocking kinetics where a molecule rarely switches between different docked states. (b)An example of memory loss after 3 h. The excitation was shut off after 500 s for 3 h to prevent dye photobleaching. Reprinted with permission from Zhuang etal., Correlating structural dynamics and function in single ribozyme molecules. Science 296 (2003) 1473-1476. Copyright 2002 AAAS. Figure 6.17 Docked states have a strong memory effect, as indicated by FRET time traces of single rlbozyme-substrate complexes, (a) Memory effect of the undocking kinetics where a molecule rarely switches between different docked states. (b)An example of memory loss after 3 h. The excitation was shut off after 500 s for 3 h to prevent dye photobleaching. Reprinted with permission from Zhuang etal., Correlating structural dynamics and function in single ribozyme molecules. Science 296 (2003) 1473-1476. Copyright 2002 AAAS.
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Ribozyme-substrate complex

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Substrate complex

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