Single cell protein Green

Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G. and Tsien, R. Y. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803-8. 

Zhang HT, Kacharmina JE, Miyashiro K, Greene MI, Eberwine J. Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution. Proc Natl Acad Sri USA 2001 98(10) 5497-5502. 

Baubet, V., Le Mouellic, H., Campbell, A.K., Lucas-Meunier, E., Fossier, R and Brulet, R (2000). Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level. Proceedings of the National Academy of Sciences, USA, 97, 7260-7265. 

In their work, the authors demonstrated the viability of cells after deposition. As the array was integrated in a microfluidic channel, docked cells could be exposed to chemicals and their responses studied. In these two examples, the authors could analyze the response of a-factor-dependent green fluorescent protein (GFP) expression cells or monitor the response of the mating mitogen-activated protein kinase (MAPK) pathway. These studies could be performed in real time and at the single cell resolution. 

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