Simple, neutral, liquid chromatography

The present article is concerned with the use of gas-liquid chromatography for neutral, basic, and acidic sugars and some of their simple derivatives. A succeeding article will treat the separation of methylated sugars and their derivatives. 

This chromatography may be used to estimate log P values. Compotmds with known log Ps are injected onto a CIS reverse-phase HPLC column and their capacity factors are used to create a calibration curve. Unknown compotmds are then injected and their capacity factors are used to predict log P. Strictly, this technique is only valid for neutral molecules. Charged molecules have more complex retention behavior than simple partition. Some liquid-liquid experiments have been reported using an octanol saturated column and an aqueous mobile... 

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