Silanisation of the Silica Surface

1 Objectives Chemical surface modification of the silica serves to  

As a result, a siloxane bond is formed and the functional group R is introduced by the organosilane. Silanisation can be performed in many ways and thus the products differ in surface chemistry, which is reflected in the chromatographic behavior. 

Depending on the surface modification, reversed-phase silicas can be grouped into (a) monomeric reversed-phase silicas chemically modified with monofunctional silanes and (b) polymeric reversed-phase silicas with a polymeric layer made by surface reaction with trifunctional silanes. 

Depending on the parent silica and the way the reversed-phase silica was modified with silanes, reversed-phase columns exhibit a distinct selectivity towards hydrophobic and polar solutes (Engelhardt, Griiner, and Scherer, 2001). 

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