Sialidase reaction mechanism

Close investigation of the reaction mechanism of influenza virus sialidases [60,320] has shown that Neu2en5Ac appears as a transition intermediate, resulting in the... 

M. Ribeirao, V. L. Pereira-Chioccola, D. Eichinger, M. M. Rodrigues, and S. Schenkman, Temperature differences for frans-glycosylation and hydrolysis reaction reveal an acceptor binding site in the catalytic mechanism of Trypanosoma cruzi trans-sialidase, Glycobiology, 1 (1997) 1237-1246. 

Another unusual glycosidase mechanism classified is encountered in family 4, which requires a unique NAD" and Mn " " assisted catalysis. Those enzymes do not follow the classical double-displacement mechanism but act through multiple catalytic steps involving oxidation-elimination-addition and reduction reactions. Other interesting examples can also be found in the case of trans-sialidases and a-glucan lyases. ... 

M. (1995) Effect of substrate aglycon on enzyme mechanism in the reaction of sialidase from influenza virus. FEBS Letters, 372, 148-150. 

Abstract In contrast to the general hydrolases, trans-sialidase from Trypanosoma cruzi (TcTS) shows excellent regio- and stereoselectivity as well as high yields in transfer reactions. Discussed are the occurrence of trans-sialidases and studies on the transfer mechanism. In detail, the preparative use by chemoenzymatic syntheses with TcTS are outlined with emphasis on the design of modified donor and acceptor substrates. Another section focuses on attempts to develop inhibitors for TcTS, and these endeavors are based on donor- and acceptor-inspired modifications as well as on some completely different structures. 

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