Si-Citrate synthase

Retey et al. (72) used this same principle in their work on the stereochemistry of citrate formation from chiral acetyl-CoA with Si-citrate synthase (Scheme 15). The chiral methyl group was converted into one of the methylene groups of succinate and the distinction between sets (I, 2, 3) and (4, 5, 6) was then based on the known different isotope effects for the removal of pro-/ ( hMd = 5.3 kH/kT = 12) vs. pro-5 (kH/kD = 1.35 kH/kj = 1.5) hydrogens of succinate in the succinate dehydrogenase reaction (73). However, the malate synthase/fumarase procedure is clearly the most commonly used method to analyze the configuration and chiral purity of chiral methyl groups. 

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