Shuffle technique

The shuffle technique consists of placing a cloth dosimeter onto the bottom of two separate platforms that are slightly bigger than a human foot. A platform... 

Initial approaches to directed evolution of enzymes rested upon the introduction of random mutations in random sites of the enzyme by the use of the error-prone PCR technique [92] or on the DNA-shuffling method [93]. Extensive research has also been reported in which every amino acid site in an enzyme was systematically subjected to saturation mutagenesis [94]. 

The reduced background of unshuffled clones is a significant advantage with these techniques, with over 75% shuffled clones in most cases and 100% with RACHITT. In addition, the higher crossover frequency observed with RACHITT, and the average of 14 per gene compared to one to four for other PCR-based methods, leads to a considerable increase in library diversity (Pelletier, 2001). 

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