Shear-Driven Micro- and Nanofluidics

DNA chips/microarray Lab-on-a-chip Micropropulsion Microscale Nanofluidic structures Nanoscale Shear-driven micro- and nanofluidics Small volume... 

Shear-Driven Micro- and Nanofluidics, Fig. 4 Bird s-eye view schematic of the rotating microchamber hybridization setup, showing the rotation stage (1) and the stainless holding shaft (2). The PVC cup (i) is used for temperature control. The etched glass consists of an outer... 

Shear-Driven Micro- and Nanofluidics, Table 1 Comparison of hybridization conditions on the shear-driven rotating microchamber system, the diffusion-driven coverslip system, and the SlidePro (GE Healthcare) for a mouse heart and spleen total RNA sample on the VIB Mouse 5KI microarray chip... 

Shear-Driven Micro- and Nanofluidics, Rgure 7 A series of different hybridization experiments done with 20 pmol (coverslip and SDH) and 40 pmol (SlidePro) of incorporated Cy3/Cy5 dyes (a) and with 5 pmol of incorporated Cy3/Cy5 dyes (b). All experiments were done with mouse heart (Cy3) versus spleen (Cy5) total RNA sample on the VIB Mouse 21 Kll microarray chip... 

Fluid flow in small devices acts differently from those in macroscopic scale. The Reynolds number (Re) is the most often mentioned dimensionless number in fluid mechanics. The Re number, defined by pUL/jj, represents the ratio of inertial forces to viscous ones. In most circumstances involved in micro- and nanofluidics, the Re number is at least one order of magnitude smaller than unity, ruling out any turbulence flows in micro/nanochannels. Inertial force plays an insignificant role in microfluidics, and as systems continue to scale down, it will become even less important. For such small Re number flows, the convective term pu Vm) of Navier-Stokes equations can be dropped. Without this nonlinear convection, simple micro/nanofluidic systems have laminar, deterministic flow patterns. They have parabolic velocity profile in pressure-driven flows, plug-like velocity profile in elec-froosmotic flows, or a superposition of both. One of the benefits from the low Re number flow is that genomic material can be transported easily without shearing in Lab-... 

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