Serine microsomal incorporation

Vandor and Richardson (1968) reported that a microsomal preparation from pea seedlings incorporated [ Clserine into lipids and that the same preparation decarboxylated the added serine. It was not clear that the serine was incorporated into phospholipid before the decarboxylation. 

Vandor and Richardson (1968) found that ethanolamine could be incorporated into lipid when incubated with a microsomal fraction from etiolated pea (P. sativum L.) seedlings. The reaction had a pH optimum of 8.5 and required Ca + for optimal activity. There was no stimulation by ATP, CTP, or glycerol-3-P. This reaction appears to be the exchange of a free base for one bound to the phospholipid not only ethanolamine, but also choline and serine could be incorporated. 

The incorporation of [ C]serine into phospholipids was catalyzed by a microsomal preparation from etiolated pea seedlings. This was apparently a base-exchange reaction (Vandor and Richardson, 1968) ... 

Fig. 2 shows that brain microsomal Ptd-Cho can act as a membrane-bound substrate for the incorporation of either ethanolamine, L-serine or choline, although with different levels of. magnitude. 

Miras, C. J., J. Mantzos, and G. Levis Incorporation of L-[3- KU] serine into microsomal phospholipids of human leucocytes. Biochim. biophys. Acta (Amst.) 84, 101—3 (1964). 

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