Serine chemical modification reagents

Under this heading might be placed many of the reactions already discussed, but there are several that fit more appropriately in such a classification. One of these, with which the author has been associated, is the formation of inactive derivatives of proteolytic enzymes by alkaline beta elimination of a derivative of the active site serine of trypsin (45) (Figure 22). This modification uses an affinity reagent followed by a second chemical modification, the alkaline beta elimination, to form the product. The products of the reaction with trypsin and chymo-... 

---