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Sequencing-by-ligation

These synthetic knots are such good substrates for type I topoisomerases that we constructed an RNA trefoil knot to seek RNA topoisomerase activity, which was suspected, but unknown. We designed the knot similarly to the DNA trefoil knot, and made the circle of the same sequence by altering the length of the linker used in the ligation. We discovered that topo HI is capable of catalyzing RNA strand-... [Pg.335]

Schouten JP, McElgunn CJ, Waaijer R, Zwijnenburg D, Diepvens F, Pals G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res 2002 30(12) e57. [Pg.638]

Restriction enzymes recognize specific recognition sequences and cut the DNA to leave cohesive ends or blunt ends. The ends of restricted DNA molecules can be joined together by ligation to create new recombinant DNA molecules. [Pg.243]

See other pages where Sequencing-by-ligation is mentioned: [Pg.33]    [Pg.110]    [Pg.112]    [Pg.47]    [Pg.54]    [Pg.55]    [Pg.59]    [Pg.59]    [Pg.121]    [Pg.71]    [Pg.73]    [Pg.33]    [Pg.110]    [Pg.112]    [Pg.47]    [Pg.54]    [Pg.55]    [Pg.59]    [Pg.59]    [Pg.121]    [Pg.71]    [Pg.73]    [Pg.564]    [Pg.396]    [Pg.119]    [Pg.8]    [Pg.570]    [Pg.155]    [Pg.3]    [Pg.10]    [Pg.311]    [Pg.310]    [Pg.91]    [Pg.91]    [Pg.98]    [Pg.216]    [Pg.78]    [Pg.109]    [Pg.109]    [Pg.406]    [Pg.61]    [Pg.65]    [Pg.167]    [Pg.217]    [Pg.502]    [Pg.649]    [Pg.171]    [Pg.101]    [Pg.169]    [Pg.1374]    [Pg.205]    [Pg.1827]    [Pg.564]    [Pg.162]    [Pg.53]    [Pg.99]    [Pg.370]   
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