Sequence-Specific Assignment Using Homonuclear 2D Spectra

7 SEQUENCE-SPECIFIC ASSIGNMENT USING HOMONUCLEAR 2D SPECTRA 

The NOESY spectrum (Fig. 12.21, right) gives the sequential connectivity, the proof that the Ser residue on the right side is followed in the primary sequence by the Val residue on the left side. On the vertical Hn = Val line we see the intraresidue crosspeaks to the Ha and Up of valine (which also appear in the TOCSY spectrum), but there is a new crosspeak that connects the of the Ser spin system (in F ) with the Hn of the Val spin system (in /Y). This crosspeak lines up with the intraresidue crosspeak on the serine Hn line (Hn = Ser in F2 and = Ser in F ) that is in the exact position of a crosspeak in the DQF-COSY 

---