Separations of compounds by partition

The analytes are separated by adsorption to a polar or nonpolar support surface or by partition into a stationary liquid phase. Silica is the most common polar adsorbent. HPLC basically involves the separation of compounds by partition on a stationary liquid phase, bonded to a support. The support, such as silica, is derivatized with a functional group that is covalently attached to the surface and is more stable than ary coated phase. Such bonded phases can be used with most solvents and buffers. A mobile liquid phase transports the sample into the column where individual compounds are selectively retained on the stationary liquid phase and thus separated. 

Outline the basis for separation of compounds by partition chromatography (Section 14.8). 

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See also in sourсe #XX -- [ , ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ , ]

See also in sourсe #XX -- [ , ]

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