Separation Techniques for Higher-Level Peaks

Having overlapping peaks in a chromatogram, the analyst has to solve the following question Which separation method is the best for separating these overlapping peaks  

The vertical separation method ( Vertical skim, drop ), separation of the tangential ( skim Tangential ), the valley-to-valley separation method, the Gauss separation method ( Gaussian skim ), or the exponential removal ( Exponential skim )  

Looking at the separation methods, you can see very quickly (just consider the vertical, the tangential, and the valley-to-valley separation method) that the separation must be faulty and full of errors.  

Overlapping peaks and overlaid curves can only be determined in exceptional cases by separating with straight lines. 

The vertical peak separation, which is called Lot method, separates peaks from each other by drawing a vertical line. 

---