Sense/antisense construct

Fig. 5. Gmhspl7.6-L antisense RNA in transgenic tobacco and levels of endogenous hs mRNAs. Sense (C) and antisense constructs (D) as depicted in Fig. 3 were used for transformation of tobacco. Total RNA was isolated from leaves of individual plants which had been subjected to heat stress (hs) for 2 h at 40 °C, or incubated at 25 °C (c). 30 pg RNA per lane were separated by electrophoresis and blots were hybridised (A) with the same gene-specific DNA probe as used in Fig. 4. This hybridisation identifies the levels of antisense RNA in individual plants Dj, D2 and D3. Hybridisation (B) of an identical blot with a single stranded antisense RNA probe representing only the inverted region of construct D (Fig. 3), shows the levels of hs-induced endogenous tobacco hsp-mRNAs. tob, untransformed tobacco plants used as a control.

Jorgensen RA, Cluster PD, English J, Que Q, Napoli CA (1996) Chalcone synthase cosuppression phenotypes in petunia flowers comparison of sense vs antisense constructs and single-copy vs complex T-DNA sequences. Plant Mol Biol 31 957-973... 

Edwards, M.E. et ah, The seeds of Lotus japonicus fines transformed with sense, antisense, and sense/ antisense galactomannan galactosyltransferase constructs have structurally altered galactomannans in their endosperm cell walls, Plant Physiol, 134,1153, 2004. 

Fig. 7. Diagrams of the schemes for modifying levels of A, alcohol dehydrogenase and B, pyruvate decarboxylase activity and testing for survival of anoxia. In A, constructs contain the 35S promoter of the cauliflower mosaic virus (35S) driving expression of the cotton Adh cDNA in either the sense (Adh) or antisense (hdA) orientation, linked to the 3 termination signal of the nopaline synthase gene (Nos). Alternatively, the expression of cotton Adh cDNA is under control of the pea Adh promoter sequence (pea Adh). In B, either the 35S promoter or the pea Adh promoter is used to drive expression of the maize pyruvate decarboxylase cDNA (Pdc), linked to a Nos 3 termination sequence. Constructs are introduced into cotton via Agrobacterium tumefaciens-mediated infection of cotton. Transformed cotton callus is then assayed for its ability to survive anoxia.

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