Binding selective

Table 11. Selective Binding of Cephalosporins to the PBPs oiE. coli ...

J Marelius, M Graffner-Nordberg, T Hansson, A Hallberg, J Aqvist. Computation of affinity and selectivity Binding of 2,4-diammopteridme and 2,4-diammoqumazolme inhibitors to di-hydrofolate reductases. J Comput-Aided Mol Des 12 119-131, 1998. 

This concept is demonstrated schematically in Figure 1.11. It can be seen that the initial bias in a system of proteins containing two conformations (square and spherical) lies far toward the square conformation. When a ligand (filled circles) enters the system and selectively binds to the circular conformations, this binding process removes the circles driving the backward reaction from circles back to squares. In the absence of this backward pressure, more square conformations flow into the circular state to fill the gap. Overall, there is an enrichment of the circular conformations when unbound and ligand-bound circular conformations are totaled. 

The described bioaffinity separations demonstrate that polyacrylamide spacers aid the selective binding of highly complex and delicate biomacromolecules and their associates. Moreover, these solutes remain biologically active after desorption probably due to the high inertness and flexibility of the surrounding polymer chains fixed on the solid support. The unbound parts of serum usually show no loss of the activities of their constituents. Thus we evaluate the surface of inorganic supports coated with chemisorbed iV-hydroxyethyl polyacrylamide and its derivatives as being biocompatible. 

Molecnles that Selectively Bind a Specific Conformation of the Protein Kinase... 

Anthraquinone, 1-hydroxy-calcium aluminum chelate compound, 1,2 metal complexes dyes, 6,86 Antiarthritis drugs labelled gold compounds, 6, 969 metal complexes, 6,758 Antibiotic M139163,2, 974 Antibiotics ionophoric, 6, 553 metal complexes selective binding, 6, 552... 

Potassium hexachloromolybdate, 3, 1230 Potassium hexacyanoferrate discovery, 1, 3 Potassium ions biology, 6, 559 selective binding biology, 6, 551... 

Sodium hexakis(formato)molybdate, 3, 1235 Sodium hypochlorite alkene epoxidation manganese catalysts, 6,378 Sodium ions biology, 6, 559 selective binding biology, 6, 551 Sodium molybdate, 3, 1230 Sodium peroxoborate, 3,101 Sodium/potassium ATPase, 6, 555 vanadate inhibition, 3, 567 Sodium pump, 6, 555 mechanism, 6, 556 Sodium pyroantimonate, 3, 265 Sodium salts... 

Fig. 42. A series of boronic acids 159-166 with chromophoric groups has been evaluated with respect to its capacity to selectively bind d-glucose or other saccharides...

In 159 and 163-166 the tertiary amine function is coordinated to the boron atom and transmits the electronic change due to the ester formation to the chromophore. In 160-162 the boron atom is directly connected to the chromophore. After the complexation of the saccharide, the change of the charge transfer, e.g., for 159 [249-251], or the fluorescence bands, e.g., for 160-166 [252-255], can be measured and interpreted. The most selective binding of n-glucose has been achieved with host 164 that forms a 1 1 complex with a macrocyclic structure (Scheme 1). 

If attachment of a particular constituent is incompatible with peptide synthesis, or detrimental to self-assembly, an alternative approach would be to add a functional group that selectively binds this molecule. After synthesis and self-assembly of the peptide, the difficult molecule could be introduced to the binding site included on the peptide. 

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