Sedimentation equilibrium buoyant density gradient

Figure 9.I. A. Profile of Ciona DNA (molecular size > 100 kb), as obtained by analytical ultracentrifugation to sedimentation equilibrium in a CsCl gradient. The 1.742 g/cm peak is the peak of the marker DNA from phage 2C. B. Fit of analytical profile of Ciona DNA (curve) with a gaussian curve (dotted line). This analysis was performed using the MacCurveFit program. The GC scale was obtained from the buoyant density values using the relationship of Schildkraut et al. (1962). (From De Luca et al., 2002).

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