Secondary isotope effects phosphate hydrolysis

Although the structure/reactivity studies conducted by Jencks and co-workers suggest that metaphosphate will not occur as an intermediate in aqueous reactions (54), and it is known that phosphorothioate esters undergo dissociative hydrolysis more rapidly than phosphate esters (40), Weiss and Cleland have recently utilized secondary 0 isotope effects to probe the mechanism of the hy-... 

The transition state of the nonenzymic reaction was also studied by Cleland and coworkers (Weiss et al., 1986). They investigated secondary 0 isotope effects on the hydrolysis of glucose 6-phosphate [2]. It was found that the isotope effect for the hydrolysis is 1.0046 per 0 substitution. 

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