Second Hypothesis Michaelis-Menten Rate Equation

1 Second Hypothesis Michaelis-Menten Rate Equation 

F re 6-9c Parity plot to test first-order rate equation for fructose isomerization using xylose isomerase derived from T. neapolitana. 

This rate equation can be linearized in several ways. One way is to simply invert both sides to obtain 

If this model fits the data, a plot of 1 /—rp versus 1/[F] will be linear, with an intercept, /, of 1 /k and a slope, S, of Km/k. In biochemistry, this type of plot is known as a Lineweaver-Burke plot Such a plot is shown in Bgure 6-9d for the data of this example. 

The Michaelis-Menten model appears to provide a much better description of the fructose isomerization data than the simple, first-order model. The points in the above graph fall very close to the best fit straight line, and the scatter seems to be random. The values of the slope and the intercept ate shown on the graph we will return to them shortly. These values were used to calculate the values of the residuals. 

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