Screening versus Confirmation

The intended use of the method must be clearly defined. A screening method is generally considered to be a method used to detect the presence of a compound, usually at or above a specific concentration. In CAC guidelines for veterinary drug residue analysis, a screening method is defined as qualitative or semi-quantitative in nature and are used as screening methods to identify the presence (or absence) of samples from a herd or lot which may 

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Fig. 6.7 Visualization of HTS data using Spotfire decision site [48]. Shown are a table of data (top left), a bar chart (top right, number of compounds screened from each plate), 3-D scatterplot (bottom left, percent inhibition in the primary assay versus percent inhibition in the confirmation assay versus percent purity), and a plate map (bottom right, well versus Column arranged by plate number). In the 3-D scatterplot and the plate map, the points are colored by plate number and sized by percent inhibition in the primary assay.

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