Screening for Polymerases with Altered Substrate Tolerance

Screening for Polymerases with Altered Substrate Tolerance 

To gain access to one or more DNA polymerases with the abilities to  

extend the terminus by addition of the next fluorophore-labeled substrate, and 

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Diverse analyses demonstrated that we prepared heteroduplex DNA fragments of 7000-9000 base pairs (bp) with complete substitution of both pyrimidine nucleotides [54]. Although the labeled DNA differs very much from natural DNA (for example, with respect to solubility, melting behavior, and circular dichroism), we 

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