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Screening and Cloning

Initial growth, screening, and cloning by limiting dilution The most popular method for the production of clones, whenever the fraction of specific antibody-secreting hybrids is lower than 10 is by limiting dilution. [Pg.74]

Li, F, Bamathan, E. S., and Karik6, K. (1994) Rapid method for screening and cloning cDNAs generated in differential mRNA display application of Northern blot for affinity capturing of cDNAs. Nucleic Acids Res. 22,1764,1765. [Pg.603]

Figure 2.3 Metagenomic cloning experiments. Isolation of genomic DNA directly from environments (soil, plants, mixed environments or thermal-vent worms are the examples Illustrated here) can recover DNA fragments which could encode for enzymes. The DNA fragments can be ligated to plasmids or DNA linkers, and then subjected to functional screening (expression cloning) and/or sequence analysis. Amplification by PCR can sometimes be used to yield libraries enriched with clones containing selected sequence motifs relating to families of enzymes... Figure 2.3 Metagenomic cloning experiments. Isolation of genomic DNA directly from environments (soil, plants, mixed environments or thermal-vent worms are the examples Illustrated here) can recover DNA fragments which could encode for enzymes. The DNA fragments can be ligated to plasmids or DNA linkers, and then subjected to functional screening (expression cloning) and/or sequence analysis. Amplification by PCR can sometimes be used to yield libraries enriched with clones containing selected sequence motifs relating to families of enzymes...
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Cloning screening

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