Schizophyllum commune cellulase from

Another source of the metal ion content of lignin is from the enzyme solutions. Two types of enzymatic preparations were used a mixture of the cellulases produced by cultures of Schizophyllum commune and Trichoderma reesei ( ) and a commercial preparation (Cellucast, Novo industry, Denmark) which is a concentrate of the cellulases produced by Trichoderma viride. Both types of enzyme preparation contain metal ions derived from the culture media. 

Trembling aspen wood (Populus tremuloides Michx.) was reduced to a powder in a Hurricane Pulverizer. Samples were prepared from extracted pulverized wood by ball-milling for 10 days in a rotating ceramic mill equipped with an air-conditioning unit which prevented the temperature of the contents of the mill from rising above ambient. Digestion of the samples with cellulase was done with a mixture of Schizophyllum commune and Trichoderma reesei culture filtrates at 30°C for 3 days. 

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