Schematic diagram fluorometer

Fig. 6.7. Schematic diagram of a single-photon timing fluorometer.

Fig. 6.10. Schematic diagram of a multi-frequency phase-modulation fluorometer. P polarizers PC Pockel s cell S sample R reference.

Fig. 5. Schematic diagram of fluorometer. Light from source 5 is rendered parallel by lens and passes through UV (or blue) filter F,. The fluorescence from sample C which may be thermostated in the metal block T passes through the cut off filter Ft and falls on the multiplier phototube MPt. In polarization measurements a polarizer Px (dichroic filter in parallel or perpendicular orientations) is inserted before the sample and the fluorescence is observed through an analyzer Pt which can be rotated with a synchronous motor.

Fig. 7.6. Schematic diagram of a phase-modulation fluorometer using a CW light source (xenon lamp or laser) and a Pockels cell as a modulator.

Figure 9.3. Schematic diagram (top view) of the components of a fluorometer (filter fluorometer or spectrofluorometer). The source is a mercury-arc or xenon-arc lamp. The excitation grating or primary filter transmits only a portion of the radiation emitted by the source. Most of the exciting radiation passes through the sample cell without being absorbed. The radiation absorbed causes the sample to fluoresce in all directions, but only the emission that passes through the aperture or slit and through the secondary filter or fluorescence grating is measured by the phototube, or photomultiplier. The output of the detector is either measured on a meter or plotted on a recorder. From G. H. Schenk, Absorption of Light and Ultraviolet Radiation, Boston Allyn and Bacon, 1973, p 260, by permission of the publisher.

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